Strawberry leaf tissues produce high levels of secondary metabolites such as polyphenols and polysaccharides, which inhibit the activity of DNA polymerases and restriction enzymes. Although rapid DNA extraction has been successfully adapted in other plant breeding programs, this method has never been reported in strawberry, which is particularly recalcitrant to DNA extraction. reported a rapid extraction method that was successfully used for PCR amplifications in various plant species, and compatible with the use of fluorescently-labeled primers for PCR fragment analysis. Alkaline (NaOH) DNA extraction has been successfully used for many important crops.
It is, therefore, critical to develop a high-throughput genotyping system that is faster and more cost-effective while still maintaining accuracy.įor effective MAS in strawberry and other Rosaceae fruit crops, a rapid DNA extraction method is necessary. Traditional cetyl trimethylammonium bromide (CTAB) DNA extraction and gel-based genotyping are becoming a bottleneck for MAS in strawberry and in other berry and fruit crops. To successfully apply MAS, it is necessary to rapidly and accurately screen a large number of individuals for multiple target traits. Thus, molecular marker techniques can be now widely applied for marker-assisted selection (MAS) in strawberry breeding. The genome of diploid strawberry ( Fragaria vesca) has recently been sequenced, rearrangement of scaffold improved, and genome scanning with sub-genome specificity is now routine. The cultivated strawberry ( Fragaria× ananassa Duchesne) is an allo-octoploid (2 n = 8 x = 56) belonging to the Rosaceae.
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